Application of genome-wide genechip for screening and identifying genes related to CD133(+)CD200(+) colorectal cancer stem cells.
- Author:
Shanshan ZHANG
1
,
2
;
Lixuan LI
;
Zaiwei HUANG
;
Xiaomin XIN
;
Bing XIAO
Author Information
- Publication Type:Journal Article
- MeSH: AC133 Antigen; Antigens, CD; genetics; metabolism; Colorectal Neoplasms; genetics; Flow Cytometry; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Glycoproteins; genetics; metabolism; Humans; Neoplastic Stem Cells; metabolism; Oligonucleotide Array Sequence Analysis; Peptides; genetics; metabolism; Transcriptome
- From: Journal of Southern Medical University 2013;33(12):1787-1791
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen and identity genes related to CD133(+)CD200(+) colorectal cancer stem cells.
METHODSThe two subpopulations of colorectal cancer cells, namely CD133(+)CD200(+) and CD133(-)CD200(-) cells, were sorted and verified by flow cytometry. The gene expression profiles of CD133(+)CD200(+)and CD133(-)CD200(-) colorectal cancer cells were examined using Affymetrix Human U133 Plus2.0 genome-wide genechip. The differentially expressed genes between the two cell subpopulations were analyzed to identify the genes responsible for the main effect in association with colorectal cancer stem cells. Real-time quantitative PCR was performed to confirm some of the differentially expressed genes identified by genechip.
RESULTSThe genechip result showed that 655 genes were differentially expressed in CD133(+)CD200(+) colorectal cancer stem cells by at least 3 folds, including 290 up-regulated and 365 down-regulated ones. Bioinformatics analysis and gene co-expression network building identified 3 genes (MDM2, PRKACG, and CACNA1G) with specific expression in CD133(+)CD200(+) colorectal cancer stem cells, and this result was confirmed by real-time quantitative PCR analysis.
CONCLUSIONA specific gene expression profile of colorectal cancer stem cells has been established through screening and identifying genes related to CD133(+)CD200(+)colorectal cancer stem cells by gene genechip technique, which provides a basis for further study of gene targeting therapy of colorectal cancer.
