Property of liposomal fusion induced by acid-sensitive polymer.
- Author:
Ru-tao WANG
1
;
Tao CHEN
;
Zhao WANG
;
Min-quan HUI
;
Jing-guo FU
Author Information
1. Faculty of Life Sciences, Northwestern Polytechnical University, Xi' an 710072, China.
- Publication Type:Journal Article
- MeSH:
Acrylates;
chemistry;
Alkylation;
Drug Carriers;
Drug Delivery Systems;
Erythrocyte Membrane;
metabolism;
Humans;
Hydrogen-Ion Concentration;
Lipids;
chemistry;
Liposomes;
chemistry;
Membrane Fusion;
Molecular Weight;
Particle Size;
Polymers;
Temperature
- From:
Acta Pharmaceutica Sinica
2008;43(9):951-955
- CountryChina
- Language:Chinese
-
Abstract:
The fusion between liposome-liposome, liposome-biomembarnes induced by acid-sensitive polymers has been systematically investigated. The polymer-liposomes were constructed by post-insertion method with the poly (2-ethylacrylic acid) (PEAA) alkylamide derivatives. The liposomal fusion was studied by use of fluorescence resonance energy transfer assay, particle size, fluorescent-photometer. The results indicated that the poly (2-ethylacrylic acid)-liposomes has very strong acidic induced fusion capability. Under acidic conditions, acid-sensitive polymer liposomes fused each other, the fusion closely related to the molecular weight of acid sensitivity polymer on the surface of liposomes. The acidic fusion of polymer-liposomes was dependent upon the lipids composition, the degree of fusion was reversely related to the cholesterol contents. Acid-en ci-nsitive polymer liposomes fused with erythrocyte ghosts. The liposomal fusion induced by acid-sensitive polymer associated with the increase of membrane permeability. The good acid-sensitivity of PEAA has been further demonstrated by membrane fusion in current experiments, and the liposomes prepared with lipid anchored-poly (2-ethylacrylic acid) were developeds s a potential pH sensitive delivery system.
