Effects of red clover extract on the activation and proliferation of mouse T lymphocytes and the NO secretion of mouse macrophages.
- Author:
Zhi YANG
1
;
Xiu-yan HUANG
;
Yao-ying ZENG
Author Information
1. Laboratory of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents;
isolation & purification;
pharmacology;
Antigens, CD;
metabolism;
Antigens, Differentiation, T-Lymphocyte;
metabolism;
CD3 Complex;
analysis;
Cell Proliferation;
drug effects;
Cells, Cultured;
Drugs, Chinese Herbal;
isolation & purification;
pharmacology;
Interleukin-2 Receptor alpha Subunit;
metabolism;
Lectins, C-Type;
Lymphocyte Activation;
drug effects;
Macrophages;
cytology;
secretion;
Male;
Mice;
Mice, Inbred BALB C;
Nitric Oxide;
secretion;
Plants, Medicinal;
chemistry;
Receptors, Transferrin;
metabolism;
T-Lymphocytes;
cytology;
drug effects;
metabolism;
Trifolium;
chemistry
- From:
Acta Pharmaceutica Sinica
2008;43(10):1019-1024
- CountryChina
- Language:English
-
Abstract:
The study investigated the effects of red clover extract (RCE) on mouse T macrophages and lymphocytes in vitro. The cell toxic effect of RCE was estimated by MTT assay. Multiple-fluorescence staining plus flow cytometry were used to detect the effect of RCE on CD69/CD25/CD71 expression of mouse T lymphocytes stimulated by Con A; CFDA-SE staining plus flow cytometry were used to analyze the effect of RCE on proliferation of T lymphocytes activated by Con A; The effect of RCE on nitric oxide (NO) secretion of mouse macrophages stimulated by lipopolysaccharide (LPS) for 24 h was assayed by Griess reagent system. We found that RCE had potent anti-inflammatory effects on mice. RCE had little cell toxic effect on mouse lymphocytes and macrophages. RCE strongly inhibited the excessive production of inflammatory mediators (NO, CD69, CD25, CD71), in a dose-dependent manner, like cyclosporine A injection. RCE could inhibit proliferation of CD3+ T lymphocytes. These data suggested that RCE might exhibit anti-inflammatory effect by inhibiting the activation and proliferation of mouse lymphocytes and the NO secretion of mouse macrophages.
