Effect of trihydroxybenzoic acid dimmer on induction of apoptosis of HL-60 cells.
- Author:
Wen-jing ZHAO
1
;
Feng-lan NIU
Author Information
1. School of Public Health, Jilin University, Changchun 130021, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
isolation & purification;
pharmacology;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Caspase 9;
metabolism;
Cytochromes c;
metabolism;
Fruit;
chemistry;
Gallic Acid;
isolation & purification;
pharmacology;
HL-60 Cells;
Humans;
Membrane Potential, Mitochondrial;
drug effects;
Mitochondria;
metabolism;
physiology;
Plants, Medicinal;
chemistry;
Reactive Oxygen Species;
metabolism
- From:
Acta Pharmaceutica Sinica
2009;44(1):42-47
- CountryChina
- Language:Chinese
-
Abstract:
Trihydroxybenzoic acid dimmer is an anti-tumor compound which is separated from water-caltrop. This study is aimed to investigate its anti-proliferation effect on HL-60 cells and the possible mechanism of inducing apoptosis. MTT assay was used to test HL-60 cells proliferation. The apoptosis, ROS levels and the mitochondrial membrane potential were detected by flow cytometry. Cytochrome c released from mitochondria to cytosol fraction was detected by Western blotting. The activity of Caspase-9 and Caspase-3 were measured by colorimetric method. It was found to inhibit HL-60 cells proliferation in a dose and time-dependent manner. Compared with control group, it caused the increase of ROS levels and a concomitant dissipation of the mitochondrial membrane potential and induced cytosolic accumulation of cytochrome c and activities Caspase-9 and Caspase-3. Therefore it can be concluded that mitochondrial-dependent pathways was involved in its induction of apoptosis of HL-60 cells.
