Isolation and structure determination of steroidal saponin from Dioscorea zingiberensis.
- Author:
De-ping XU
1
;
Chang-ying HU
;
Lin WANG
;
Xiao-chun WANG
;
Zi-jie PANG
Author Information
1. School of Food Science and Technology, Jiangnan University, Wuxi 214036, China. xdp1219@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Dioscorea;
chemistry;
Molecular Structure;
Plants, Medicinal;
chemistry;
Rhizome;
chemistry;
Saponins;
chemistry;
isolation & purification
- From:
Acta Pharmaceutica Sinica
2009;44(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
To study the chemical constituents of Dioscorea zingiberensis Wright, the EtOH extract of fresh rhizomes of D. zingiberensis was concentrated and partitioned further to produce petroleum ether-, ethylacetate-, n-butanol- and water-soluble fractions. The water-soluble fraction was subjected to column chromatography on macro resin AB-8, and the final products were obtained by repeated reversed-phase ODS and MCI gel CHP 20P column chromatography. Structures of compounds were elucidated by 1H NMR, 13C NMR, 135DEPT, HMQC, HMBC and TOCSY spectroscopic analyses. A new steroidal saponin was isolated, which was identified as (25R)-26-O-(beta-D-glucopyranosyl)-furost-5-en-3 beta, 16, 20, 26-tetraol-22-seco-3-O-beta-D-glucopyranosyl-(1--> 3)-beta-D-glucopyranosyl-(1--> 4)-[alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside. The compound is a novel skeletally steroidal saponin, named as zingiberenin F (1). It was reported for the first time from D. zingiberensis Wright.
