Optimization on the production of analgesic peptide from Buthus martensii Karsch in Pichia pastoris.
- Author:
Jin-ling YANG
1
;
Hui-xia HE
;
Hui-xin ZHU
;
Ke-di CHENG
;
Ping ZHU
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Amino Acids;
pharmacology;
Analgesics;
isolation & purification;
metabolism;
Animals;
Fermentation;
Gene Expression;
Hydrogen-Ion Concentration;
Methanol;
pharmacology;
Peptides;
isolation & purification;
metabolism;
Pichia;
genetics;
metabolism;
Recombinant Proteins;
genetics;
metabolism;
Scorpion Venoms;
genetics;
isolation & purification;
metabolism;
Scorpions;
chemistry
- From:
Acta Pharmaceutica Sinica
2009;44(1):91-94
- CountryChina
- Language:Chinese
-
Abstract:
The technology of liquid fermentation for producing the recombinant analgesic peptide BmK AngM1 from Buthus martensii Karsch in Pichia pastoris was studied by single-factor and orthogonal test. The results showed that the optimal culture conditions were as follows: 1.2% methanol, 0.6% casamino acids, initial pH 6.0, and three times of basal inoculation volume. Under the above culture conditions, the expression level of recombinant BmK AngM1 in Pichia pastoris was above 500 mg x L(-1), which was more than three times of the control. The study has laid a foundation for the large-scale preparation of BmK AngM1 to meet the needs of theoretical research of BmK AngM1 and development of new medicines.
