Differential expressions of microRNAs and their predicted targets in liver transplant recipients with long-term stable survival.
- Author:
Kebo ZHONG
1
;
Peng ZHANG
;
Xiaoshun HE
;
Xiaofeng ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers; metabolism; Case-Control Studies; Down-Regulation; Graft Rejection; Humans; Leukocytes, Mononuclear; metabolism; Liver Transplantation; MicroRNAs; metabolism; Oligonucleotide Array Sequence Analysis; Real-Time Polymerase Chain Reaction; Survival Rate; Transforming Growth Factor beta
- From: Journal of Southern Medical University 2015;35(11):1557-1563
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) differs between liver transplant recipients with long-term stable survival and those with acute rejection.
METHODSTwenty-nine liver transplant recipients with long-term stable survival (STA) group, 10 recipients with acute rejection (RJ group), and 17 healthy subjects (control group) were recruited for genome-wide microarray analysis of miRNA expressions in the PBMCs. The differentially expressed miRNAs among the 3 groups were validated by real-time PCR, and the targets of these miRNAs were predicted.
RESULTSCompared with the RJ group, the STA group showed down-regulation of 13 miRNAs in the PBMCs. Of these down-regulated miRNAs, miRNA-18b, miRNA-340 and miRNA-106b were validated by real-time PCR, and the latter two miRNAs were predicted to target the TGF-β pathway.
CONCLUSIONSThe differentially expressed miRNAs in liver transplant recipients with long-term stable survival, namely miRNA-18b, miRNA-340 and miRNA-106b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.
