Combined detection of thyroid transcription factor 1 and napsin A in pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma.
- Author:
Xiaoyan XU
1
;
Hongwei LIU
;
Huang JIANG
;
Chuan LI
;
Shuhui YUAN
;
Jinhua YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; diagnosis; metabolism; Aspartic Acid Endopeptidases; metabolism; Biomarkers, Tumor; metabolism; Humans; Immunohistochemistry; Lung Neoplasms; diagnosis; metabolism; Nuclear Proteins; metabolism; Pleural Effusion; Sensitivity and Specificity; Thyroid Nuclear Factor 1; Transcription Factors; metabolism
- From: Journal of Southern Medical University 2015;35(11):1610-1613
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the value of detecting thyroid transcription factor 1 (TTF-1) and Noval aspartic proteinase of pepsin family A (napsin A) in pleural fluid cell blocks in cytopathologic diagnosis of pulmonary adenocarcinoma.
METHODSConventional cell smears of pleural effusions were obtained from 48 patients with a history of lung adenocarcinoma for cytological analysis. The cell blocks were prepared using the cytological specimens and examined with immunohistochemistry for TTF-1 and napsin A. The rates of a positive diagnosis of pulmonary adenocarcinoma were compared between the two methods, and the diagnositic value of TTF-1 and napsin A in pleural fluid cell blocks was evaluated.
RESULTSImmuno- histochemistry of the cell block sections yielded a significantly higher positive rate of diagnosis than cytological analysis of conventional cell smear (84.44% vs 55.56%, P<0.05). Most of the pleural fluid cell blocks showed positive expressions of TTF-1 (36/38, 94.74%) and napsin A (30/38, 78.95%), and none of samples showed TTF-1 or napsin A expression in the mesothelial cells (P<0.05). The combination detection of TTF-1 and napsin A in pleural fluid cell blocks had a high diagnosis value with a diagnostic sensitivity of 97.37% and a specificity of 100% for pulmonary adenocarcinoma.
CONCLUSIONSThe combined detection of TTF-1 and napsin A in pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma.
