Effects of tumor necrosis factor-α on release of MMP-3, MMP-9, and interleukin-17 in mouse bone marrow-derived mast cells in vitro.
- Author:
Yujiao CHEN
1
;
Qingqing OUYANG
;
Ran WANG
;
Jing WU
;
Jinjun ZHAO
;
Min YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; metabolism; Cells, Cultured; Dose-Response Relationship, Drug; Interleukin-17; metabolism; Mast Cells; metabolism; Matrix Metalloproteinase 3; metabolism; Matrix Metalloproteinase 9; metabolism; Mice; RNA, Messenger; Real-Time Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; pharmacology
- From: Journal of Southern Medical University 2015;35(11):1633-1637
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of tumor necrosis factor-α (TNF-α) on the release of matrix metalloproteinase-3 (MMP-3), MMP-9, and interleukin-17 (IL-17) in cultured mouse bone marrow-derived mast cells (BMMCs) in vitro.
METHODSPrimarily cultured mouse BMMCs at 8 weeks were exposed PBS (control) or TNF-α at the concentrations of 2, 10, or 50 ng/mL for 12 or 24 h. Real-time PCR was performed to detect the mRNA expressions of MMP-3, MMP-9, and IL-17 in the exposed cells.
RESULTSA 12-hour exposure of the BMMCs to TNF-α caused significantly increased expressions of MMP-3, MMP-9, and IL-17 in a concentration-dependent manner (P<0.05). Prolonged exposures of the cells to 2 and 10 TNF-α for 24 h further increased MMP-3, MMP-9, and IL-17 mRNA expressions, but exposure to 50 ng/mL TNF-α for 24 h increased only MMP-3 and MMP-9 expressions but not IL-17 mRNA expression.
CONCLUSIONSTNF-α treatment of primarily cultured BMMCs can significantly increase the cellular expressions of MMP-3, MMP-9, and IL-17 mRNA in a time- and dose-dependent manner.
