Effect of the hydrophobin HFBI-fusion tag on exogenous protein accumulation in tobacco plant.
- Author:
Xiqian ZHANG
1
;
Hongzhen MU
;
Ting MA
;
Xiangzhen DING
;
Zhiying LI
;
Sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Endoplasmic Reticulum; Genetic Engineering; methods; Genetic Vectors; Green Fluorescent Proteins; biosynthesis; Imidazoles; chemistry; Plant Leaves; metabolism; Plants, Genetically Modified; genetics; metabolism; Recombinant Fusion Proteins; biosynthesis; Tobacco; genetics; metabolism
- From: Journal of Southern Medical University 2015;35(12):1665-1671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanisms by which HFBI fusions increase recombinant fusion protein accumulation in plants.
METHODSThe HFBI sequence from Trichoderma reesei was synthesized and two plant expression vectors for expression of green fluorescence protein (GFP) and GFP-HFBI were constructed. The vectors were inoculated in Nicotiana benthamiana plants through agroinfiltration, and the expression levels and mRNA accumulation levels of GFP in Nicotiana leaves were examined by Western blotting, ELISA and RT-PCR.
RESULTSThe HFBI fusion tag significantly enhanced the accumulation of GFP in the leaves of N. benthamiana without causing toxic effects. Endoplasmic reticulum-targeted GFP-HFBI fusion induced the formation of spherical protein particles in the plant cells.
CONCLUSIONHFBI fusions can increase the accumulation of its fusion partner in plants by forming stable protein particles, which probably shields the target protein from endogenous protease-induced degadation. HFBI fusion technology provides an alternative to improving recombinant protein expression in plants from agroinfection-compatible expression vectors.
