Effect of Astragalus mongholicus polysaccharides on gene expression profiles of dendritic cells isolated from healthy donors.
- Author:
Chaojun CHEN
1
;
Qiang FU
;
Yuejun LI
;
Zhiliang LI
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus Plant; chemistry; CD36 Antigens; metabolism; Cell Differentiation; Dendritic Cells; drug effects; Humans; Interleukins; metabolism; Nuclear Proteins; metabolism; Phosphoproteins; metabolism; Polysaccharides; pharmacology; Transcriptome
- From: Journal of Southern Medical University 2015;35(12):1802-1805
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-atherosclerosis mechanism of Astragalus mongholicus polysaccharides (APS) by examining its effect on gene expression profiles of the dendritic cells (DCs) from healthy donors.
METHODSPeripheral blood DCs from healthy donors were incubated with 200 mg/L APS overnight, and changes in the gene expression profiles were investigated using microarray technique and RT-PCR.
RESULTSCompared with the control cells, APS-treated DCs showed significantly up-regulated expressions of CD36 (0.97 ± 0.23 vs 5.45 ± 1.14) and IL-27 (1.08 ± 0.22 vs 2.97 ± 0.61) and down-regulated expression of expression of IFI16 (0.98 ± 0.18 vs 0.46 ± 0.11).
CONCLUSIONSAPS can promote the maturation and differentiation of DCs by up-regulating CD36 and IL-27 and down-regulating IFI16, and thus positively affects the occurrence and progression of the atherosclerosis.
