Expression pattern of membrane type-1 matrix metalloproteinase in primary breast carcinomas.
- Author:
Guangyu YAO
1
;
Lujia CHEN
;
Xiaolei HU
;
Wenji LI
;
Changsheng YE
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; genetics; metabolism; Female; Humans; Immunohistochemistry; In Situ Hybridization; Matrix Metalloproteinase 14; genetics; metabolism; RNA, Messenger; genetics; metabolism; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2016;36(1):94-97
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the expression pattern of membrane type-1 matrix metalloproteinase (MT1-MMP) in breast carcinomas.
METHODSForty-three breast cancer tissues were collected and examined for MT1-MMP protein and mRNA expressions using immunohistochemistry, semi-quantitative RT-PCR and in situ hybridization.
RESULTSImmunohistochemistry of the breast cancer specimens showed MT1-MMP immunoreactivity on the cancer cell membrane. MT1-MMP mRNA was located in the stromal cells surrounding the breast cancer nest as shown by in situ hybridization. MT1-MMP mRNA expression was detected in all of the carcinomas, but its level was significantly lower in immunohistochemically negative specimens than in positive ones (0.547=0.0886 vs 0.759=0.0802, Plt;0.01).
CONCLUSIONMT1-MMP is very likely produced by stromal cells surrounding the breast cancer nest and anchored on the cell membrane after activation.
