Genome-wide profiling of alternative polyadenylation in mouse female germline stem cells.
- Author:
Ting-Ting SHEN
1
;
Xiao-Li ZHANG
;
Pan ZHANG
;
Ya-Ni KANG
;
Jing TIAN
;
Xiao-Dong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: 3' Untranslated Regions; Animals; Cell Differentiation; Embryonic Germ Cells; metabolism; Embryonic Stem Cells; metabolism; Female; Gene Expression Regulation; Genome; Mice; Polyadenylation
- From: Journal of Southern Medical University 2016;36(2):157-162
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo perform a genome-wide alternative polyadenylation (APA) profiling in both mouse female germline stem cells (FGSCs) and embryonic stem cells (ESCs) and explore the role of germline-specific APA in the biological behaviors of FGSCs.
METHODSWe used a high-throughput sequencing-based method 3T-Seq to profile the genome-wide 3' termini of the transcripts and delineate all the APA sites in mouse FGSCs and ESCs. The genes with altered APA sites in FGSCs compared with ESCs were analyzed with DAVID Gene Ontology tool for their biological roles.
RESULTSWe identified a total of 50243 APA sites in 16973 genes. In FGSCs, 1148 genes were shown to have alterations in 3'UTR length, among which 795 ( 66%) genes had shortened and 353 (34%) had lengthened 3'UTR. Some of the genes with shortened 3'UTR were involved in germ cell development.
CONCLUSIONSOur genome-wide APA profiling analysis reveals a cell type-specific APA alternation in FGSCs, and APA-mediated 3'UTR alteration contributes to germline-related biological process. This study provides a framework for understanding the post-transcriptional regulation mechanisms in FGSCs.
