Inhibitory effect of Biejiajian pills on HepG2 cell xenograft growth and expression of β-catenin and Tbx3 in nude mice.
- Author:
Bin WEN
1
;
Hai-Tao SUN
;
Song-Qi HE
;
Lei LA
;
Hai-Yan AN
;
Jie PANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Carcinoma, Hepatocellular; drug therapy; metabolism; Cell Proliferation; Drugs, Chinese Herbal; pharmacology; Hep G2 Cells; Humans; Liver Neoplasms; drug therapy; metabolism; Mice; Mice, Nude; Proliferating Cell Nuclear Antigen; metabolism; T-Box Domain Proteins; metabolism; Wnt Signaling Pathway; Xenograft Model Antitumor Assays; beta Catenin; metabolism
- From: Journal of Southern Medical University 2016;36(2):210-214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular mechanism by which Biejiajian pills inhibit hepatocellular carcinoma in a nude mouse model bearing HepG2 cell xenograft.
METHODSThe inhibitory effect of Biejiajian pills on the growth of HepG2 cell xenograft in nude mice was observed. Immunohistochemical method was used to examine proliferating cell nuclear antigen (PCNA) expression in HepG2 cell xenograft, and TUNEL method was employed to detect the cell apoptosis; the expression levels of β-catenin and Tbx3 were measured by Western blotting.
RESULTSBiejiajian pills significantly suppressed the growth of HepG2 cell xenograft in nude mice. The tumor-bearing mice treated with a high and a moderate dose of Biejiajian pills showed significantly increased apoptosis rate of the tumor cells [(22.9±1.220)% and (14.7±0.50)%, respectively] compared with the control group [(5.5±0.90)%, P<0.05]. Treatment with Biejiajian pills significantly decreased the expressions of PNCA, β-catenin, and Tbx3 in the cell xenograft (P<0.05).
CONCLUSIONSBiejiajian pills can inhibit the growth of HepG2 cell xenograft in nude mice and promote tumor cell apoptosis possibly by inhibiting PNCA expression and the Wnt/β-catenin signaling pathway.
