Sequence analysis on measles viruses isolated in Shanghai in 2005.
- Author:
Shu-hua LI
1
;
Zheng NI
;
Li-wen JU
;
Hui-guo SHEN
;
Yi-yun TAN
;
Lu-fang JIANG
;
Lian-di ZHOU
;
Yu-zun LIN
;
Ying-jie ZHENG
;
Qing-wu JIANG
Author Information
- Publication Type:Journal Article
- MeSH: China; epidemiology; Disease Outbreaks; Genotype; Humans; Measles; epidemiology; genetics; Measles virus; genetics; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Epidemiology 2007;28(2):165-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo ascertain the genetic characterization and genotype of measles viruses isolated in Shanghai region, in 2005.
METHODSMeasles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus of nucleprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain published in GenBank.
RESULTS4 measles viruses were isolated from 10 throat swab specimens, and the sequence analysis indicated that they belonged to H1 genotype. The homogeneity of 450 nucleotides in the C terminal of the N gene was at 98%-98.2% as compared to H1 genotype (China93-7). They differed from genotype H2 (China94-1) at 6.4%-6.9% and from genotype A (Edmonston) at 6.7%-6.9%, from measles vaccine (Shanghail91) at 7.6%-8.0%. They differed from the other measles viral strain isolated in China in 1993 - 2005 at 0.2%-3.7%. The variation within 4 isolated measles viruses was at 0.7%-1.3%.
CONCLUSIONIt was H1 genotype measles viruses,which are the native viruses in China that led to the outbreak of measles in Shanghai, in 2005.
