Genome-wide linkage scan for an ethnic Han Chinese pedigree affected with schizophrenia.
- VernacularTitle:一个中国汉族精神分裂症高发家系的全基因组连锁分析
- Author:
Lian-sheng ZHAO
1
;
Ying-cheng WANG
;
Jin-xue WEI
;
Xiang LIU
;
Zhen-xing YANG
;
Tao LI
;
Xiao-gang CHEN
;
Xiao-hong MA
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; China; Chromosomes, Human, Pair 12; Chromosomes, Human, Pair 4; Female; Genetic Linkage; Genome-Wide Association Study; Genotype; Humans; Lod Score; Male; Pedigree; Polymorphism, Single Nucleotide; Schizophrenia; genetics
- From: Chinese Journal of Medical Genetics 2013;30(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo perform genome-wide linkage analysis for an ethnic Han Chinese pedigree with schizophrenia in order to locate the susceptibility genes.
METHODSGenomic DNA was extracted from 4 mL of peripheral blood using conventional phenol-chloroform method. Illumina Infinium Linkage 24 BeadChips chip was used for determining the genotypes through detection of single nucleotide polymorphisms (SNPs). After processing the raw data using Illumina BeadStudio software, two-point nonparametric linkage analysis and two-point parametric linkage analysis were performed with Merlin software.
RESULTSBy two-point nonparametric linkage analysis, 27 sites with high LOD scores (LOD=0.63-0.75, P U+003C 0.05) were identified. Among these, 3 SNPs(rs993694, rs992690 rs1861577) were located in 12p12.3 region, whilst the remainders were located in 4p12-q22 region. Two-point parametric linkage analysis under a dominant model has yielded almost identical results.
CONCLUSIONChromosomal regions 4p12-q22 and 12p12.3 probably contain susceptibility genes for schizophrenia.
