Genetic polymorphism of human immunodeficiency virus coreceptor CCR5Delta32 and CCR2-64I alleles in Chinese Yi Ethnic group in Sichuan.

Xiao-ling Deng; Kun-xue Hong; Jian-ping Chen; Yu-hua Ruan; Ming-yan XU; Guang-ming Qin; Ke LI; Hui Xing; Yi-ming Shao

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Genetic polymorphism of human immunodeficiency virus coreceptor CCR5Delta32 and CCR2-64I alleles in Chinese Yi Ethnic group in Sichuan.

Author: Xiao-Ling DENG ¹ ; Kun-Xue HONG ; Jian-Ping CHEN ; Yu-Hua RUAN ; Ming-Yan XU ; Guang-Ming QIN ; Ke LI ; Hui XING ; Yi-Ming SHAO
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1. National Center for Sexually Transmitted Disease and Acquired Immune Deficiency Syndrome Control and Prevention, Chinese Center for Disease Centrol and Prevention, Beijing 100050, China.
Publication Type:Journal Article
MeSH: Adult; Alleles; Base Sequence; China; ethnology; Female; HIV Infections; virology; HIV-1; genetics; Humans; Male; Middle Aged; Molecular Sequence Data; Polymorphism, Genetic; Receptors, CCR5; genetics; Receptors, HIV; genetics
From: Chinese Journal of Epidemiology 2004;25(12):1050-1053
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore genetic polymorphisms CCR5 of HIV coreceptor and CCR2 in Chinese Yi Ethnic group in Sichuan.
METHODSGenomic DNA samples were obtained from 119 healthy individuals and 88 HIV-1 infected individuals of Chinese Yi Ethnic group in Sichuan. Polymerase chain reaction (PCR), cloning and gene sequencing techniques were employed to identify the genotype of CCR5Delta32; PCR-restriction fragment length polymorphism (RFLP) and gene sequencing were employed to identify the CCR2-64I alleles.
RESULTSAt CCR5 locus, 2 heterozygotes (CCR5-wt/Delta32) and none homozygote (CCR5-Delta32/Delta32) were observed in 119 healthy individuals, allelic frequency of CCR5-Delta32 was 0.84%; No mutant was found in 88 HIV-1 infected individuals. At CCR2 locus, 26 heterozygotes (CCR2-64V/64I) and two homozygotes (CCR2-64I/64I) were observed in healthy individuals but the allelic frequency CCR2-64I was 12.61%. Among infected individuals, 12 heterozygotes (CCR2-64V/64I) and 7 homozygotes (CCR2-64I/64I) were observed and the allelic frequency CCR2-64I was 13.27%. Statistical analysis revealed that the differences of both loci between healthy and infected individuals were insignificant. Both loci were consistent with the Hardy-Weinberg equilibrium in the two different groups.
CONCLUSIONThe polymorphism of CCR5Delta32 and CCR2-64I alleles from Chinese Yi Ethnic group was detected which was of significance for the evaluation of genetic resistance to HIV-1 infection in Chinese population.