The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus.
- Author:
Ming WANG
1
;
Yang GAO
;
Duan-hua ZHOU
;
Xin-wei WU
;
Xiao-shuang CHEN
;
Biao DI
;
Yu-fei LIU
;
Fang CHEN
;
Lin DU
;
Hui-fang XU
;
Jing GU
;
Bo-jian ZHENG
;
Jian-guo XU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Nucleocapsid Proteins; blood; Polymerase Chain Reaction; RNA, Viral; blood; Reagent Kits, Diagnostic; standards; SARS Virus; isolation & purification; Severe Acute Respiratory Syndrome; diagnosis; virology
- From: Chinese Journal of Epidemiology 2005;26(1):22-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients.
METHODSThree enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA.
RESULTSIn 162 serum samples, 90.2% (55/61) became N protein positive in 1 - 5 days and 92.8% (13/14) became positive IgM and IgG in 15 - 18 days after the onset of disease, respectively. On 82 gorgling samples, the positive rates of F-PCR were 56.3% (14/24) in 1 - 5 days and 71.4% (10/14) in 6 - 9 days after the onset.
CONCLUSIONOther than F-PCR, N protein had good effect in the early detection on dubious patients which could lead to effective prevention and control of the epidemic.
