Study on the RNA of severe acute respiratory syndrome (SARS) associated coronavirus in the blood and excretion of convalescent patients with SARS.
- Author:
Zhao-rui CHANG
1
;
Ren-quan YANG
;
Yan-bin WANG
;
Li-li REN
;
Min WANG
;
Yao-wu YANG
;
Li GUO
;
Yan-gong CHAO
;
Cheng-yi QU
;
Jian-wei WANG
;
Tao HONG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Convalescence; Feces; virology; Female; Humans; Male; Middle Aged; RNA, Viral; analysis; blood; Reverse Transcriptase Polymerase Chain Reaction; SARS Virus; isolation & purification; Severe Acute Respiratory Syndrome; virology; Sputum; virology
- From: Chinese Journal of Epidemiology 2005;26(3):201-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the RNA of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the blood and excretion of convalescent patient with SARS for prevention and treatment of the disease.
METHODSA total of 276 samples, including plasma, urine, feces and sputum, obtained from 23 convalescent patients with SARS were studied at 3 time-points at least 21 days after the onset of symptoms. RNA was extracted and nested reverse transcription-polymerase chain reaction (RT-PCR) was carried out using SARS-CoV specific primers.
RESULTSAmong the 276 samples, SARS-CoV RNA was detected in 6 cases (38.8%) by nested RT-PCR. The positive rates of SARS-CoV RNA was 5.8% in feces and 2.9% in sputum samples but SARS-CoV RNA was not detectable in plasma and urine of all the cases.
CONCLUSIONThe existence of SARS-CoV RNA in the excretion of some convalescent patients with SARS showed that the excretion from these patients should be carefully treated whilthe re-transmission of SARS by which, should be further studied.
