Effects of interferon-gamma and tumor necrosis factor-alpha on the fertilizing capacity of human sperm and their mechanisms.
- Author:
Shu-Ling BIAN
1
;
Hong-bo JIN
;
Shu-zhen WANG
;
Hai-feng ZHANG
;
Chang-ren GU
;
Wei ZHANG
;
Xiao-guang YU
Author Information
- Publication Type:Journal Article
- MeSH: Acrosome Reaction; drug effects; Adult; Calcium-Transporting ATPases; metabolism; Chromatography, High Pressure Liquid; Humans; Interferon-gamma; pharmacology; Male; Middle Aged; Nitric Oxide; metabolism; Sodium-Potassium-Exchanging ATPase; metabolism; Spermatozoa; drug effects; enzymology; metabolism; Superoxide Dismutase; metabolism; Tumor Necrosis Factor-alpha; pharmacology
- From: National Journal of Andrology 2007;13(8):681-684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of interferon-gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-gamma) on the sperm acrosin activity and the rate of acrosome reaction and to probe into their mechanisms.
METHODSThirty-six nearly normal semen samples were treated with IFN-gamma and/or TNF-alpha after isolated by 75% Percoll. The sperm acrosin activity was tested by the method of BAEE/ADH Unity, the rate of acrosome reaction observed by Triple-stain technique, the NO concentration measured by HPLC and the activities of Na+ -K+ -ATPase, Ca2+ -ATPase and SOD assayed by kit method.
RESULTSBoth IFN-gamma and TNF-gamma could decrease sperm acrosin activity and acrosome reaction (P < 0.05 or P < 0.01). TNF-alpha showed stronger inhibiting effect, IFN-gamma markedly reduced the activities of Na+ -K+ -ATPase, Ca2+ -ATPase and SOD in sperm (P < 0.01), and their synergistic action was weaker. However TNF-alpha produced hardly any effect on Na+ -K+ -ATPase and Ca2+ -ATPase. The NO concentration in sperm was significantly increased by IFN-gamma and/or TNF-alpha (P < 0.01).
CONCLUSIONIFN-gamma and TNF-alpha have some inhibiting effect on sperm acrosin activity and the rate of acrosome reaction, which could be attributed to their influence on the activities of Na+ -K+ -ATPase, Ca2+ -ATPase and SOD, the NO concentration and so on.
