Changes in the expression of voltage-dependent ca2+ channels in asthenospermia.

Author: Lu LI ¹ ; Ji-hong LIU
Author Information

1. Department of Urology, Tongji Hospital, Tongii Medical College, Huazhong University of Science & Technology, Wu-han, Hubei 430030, China.
Publication Type:Journal Article
MeSH: Asthenozoospermia; genetics; pathology; Calcium Channels; genetics; Gene Expression; Humans; Male; Protein Isoforms; genetics; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Semen; metabolism; Sperm Motility; genetics
From: National Journal of Andrology 2007;13(8):706-709
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the relationship between the expression of voltage-dependent Ca2+ channels (VDCCs) cal mRNA and asthenospermia.
METHODSBased on the WHO criteria, we filtered the donated semen by computer-aided sperm analysis (CASA), optimized the ejaculated sperm by discontinuous Percoll grads centrifugation, and examined and quantitated the multitype VDCCs alpha1 mRNA expressions in the sperm of normal men and asthenospermia patients using reverse transcription polymerase chain reaction (RT-PCR).
RESULTSCompared with the normal sperm, there were significant differences among the expressions of alpha1B (39.40 +/- 9.47), alpha1C (48.30 +/- 11.60), alpha1E (3.20 +/- 0.78), alpha1G (8.40 +/- 2.03) and alpha1H (5.70 +/- 1.47) mRNA messages in asthenospermia (P < 0.01).
CONCLUSIONThe abnormal expression of L-type and/or non-L-type VDCCs' mRNAs may be one of the causes of asthenospermia.