Inhibition of telomerase with hTERT antisense enhances TNF-alpha-induced apoptosis in prostate cancer cells PC3.
- Author:
Xiao-dong GAO
1
;
Yi-rong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line, Tumor; Cell Survival; drug effects; Enzyme-Linked Immunosorbent Assay; Humans; Male; Oligodeoxyribonucleotides, Antisense; genetics; pharmacology; Polymerase Chain Reaction; Prostatic Neoplasms; enzymology; genetics; pathology; Telomerase; antagonists & inhibitors; genetics; metabolism; Tumor Necrosis Factor-alpha; pharmacology
- From: National Journal of Andrology 2007;13(8):723-726
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibiting effect of telomerase with hTERT antisense on TNF-alpha-induced apoptosis in prostate cancer cells PC3.
METHODSAntisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) and telomerase PCR-ELISA Kit, cell viability was determined by MTT assay, and cell apoptosis was observed by morphological method and determined by flow cytometry.
RESULTSAS PS-ODN could significantly inhibit the telomerase activity and increase the susceptibility of TNF-alpha-induced apoptosis of PC3 cells.
CONCLUSIONInhibition of telomerase with hTERT antisense can enhance TNF-alpha-induced apoptosis in prostate cancer cells.
