siRNA knocks down the expression of AR gene in PC3 cell lines.
- Author:
Wei CHEN
1
;
Bin YU
;
Dan XIE
;
Ling-wu CHEN
;
Jun-hang LUO
;
Jian LU
;
Yu-ping DAI
;
Ke-li ZHENG
;
Hua MEI
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line, Tumor; Genetic Vectors; genetics; Humans; Male; Prostatic Neoplasms; genetics; metabolism; pathology; RNA Interference; RNA, Small Interfering; genetics; Receptors, Androgen; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; methods; Transfection
- From: National Journal of Andrology 2007;13(9):780-783
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the suppression of the expression of androgen receptor (AR) gene in PC3 cells after AR-specific siRNAs transfection, and to search for the siRNA (s) with the greatest suppressing efficiency.
METHODSFive AR-specific siRNAs were selected, RNAi expression vectors were constructed and transfected into PC3 cells, and the AR expression was detected by real time FQ-PCR and Western blot. A nonsense small RNA was set as negative control.
RESULTSCompared with the control group, the AR expression decreased in various degrees in the 5 experimental groups (P < 0.05), and siRNA1, siRNA4 and siRNA5 showed the greatest suppressing efficiency as compared with the other experimental groups, with statistically significant difference (P < 0.05).
CONCLUSIONThe AR-specific siRNAs could suppress the endogenous expression of target gene. Three siRNAs with great suppressing efficiency were identified and the expression vectors were constructed successfully. It can be applied in the future researches in vivo.
