Determination of alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer.
- Author:
Jin-chun LU
1
;
Hui-ru XU
;
Yu-feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biochemistry; instrumentation; methods; Humans; Male; Reference Values; Reproducibility of Results; Semen; enzymology; alpha-Glucosidases; analysis; metabolism
- From: National Journal of Andrology 2007;13(9):791-794
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method of determining alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer.
METHODSAlpha-glucosidase activity in seminal plasma from 51 men with normal semen parameters in routine semen analysis were detected by semi-automatic biochemistry analyzer and manual glucose oxidase method, respectively. Then, the intra-assay and inter-assay coefficient variation (CV) and normal reference value were calculated. In the meanwhile, the correlation between the two methods was analyzed.
RESULTSThe intra-assay CVs of 2 seminal plasma samples with different alpha-glucosidase activity detected by semi-automatic biochemistry analyzer were 12.63% and 9.13%, and the inter-assay CVs were 10.67% and 13.49%, respectively. The normal reference value for seminal alpha-glucosidase activity detected with semi-automatic biochemistry analyzer ranged from 102.28 to 555.08 U/L. There was a significantly positive correlation between the semi-automatic biochemistry analyzer and the manual glucose oxidase method (r = 0.792, P < 0.01).
CONCLUSIONThe method of determining alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer, with its simplicity, less cost of time and reagents, and more reliable result, could be applied to clinical laboratory medicine.
