PDCD5 induces the apoptosis of human prostate cancer cells PC-3M-1E8.
- Author:
Shu-jun LI
1
;
Jing YU
;
Xue-fei ZHAO
;
Ying JIANG
;
Zi-jun LIU
;
Xiao-guang YU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; genetics; physiology; Apoptosis Regulatory Proteins; genetics; physiology; Cell Line, Tumor; Humans; In Situ Nick-End Labeling; Lipids; chemistry; Male; Neoplasm Proteins; genetics; physiology; Plasmids; chemistry; genetics; Prostatic Neoplasms; genetics; pathology; Reverse Transcriptase Polymerase Chain Reaction; Transfection; methods
- From: National Journal of Andrology 2007;13(11):979-982
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis-promoting effect of PDCD5 on human prostate cancer cells PC-3M-1E8.
METHODSPCI-neo and PCI-neo-PDCD5 were transfected into PC-3M-1E8 cells by Lipofectamine 2000, the viability of the cells was analyzed by MTT assay 16 hours after removal of the serum, and the apoptosis was determined by in situ end-labeling and electron microscopy.
RESULTSThe viability and growing speed of the transfected cells were significantly decreased and their apoptotic indexes significantly increased as compared with the control group (P < 0.001).
CONCLUSIONPDCD5 may significantly inhibit the in vitro growth and promote the apoptosis of human prostate cancer cells PC-3M-1E8.
