Over-expression of hypoxia-inducible factor-1 alpha increases angiogenesis of LNCaP cells.
- Author:
Yi-li HAN
1
;
Da-lin HE
;
Yong LUO
;
He-peng CHENG
;
Guang-feng ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line, Tumor; Fluorescent Antibody Technique; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; genetics; metabolism; Lipids; chemistry; Male; Nitric Oxide Synthase; metabolism; Plasmids; chemistry; genetics; Prostatic Neoplasms; blood supply; metabolism; pathology; Transfection; methods; Vascular Endothelial Growth Factor A; metabolism
- From: National Journal of Andrology 2007;13(11):988-991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of hypoxia-inducible factor-1 alpha (HIF-la) on angiogenesis in human prostate cancer cells.
METHODSHuman prostate cancer cells of the line LNCaP were cultured and transfected by the recombinant plasmid pcDNA3. 1(-)/HIF-1alpha containing the gene HIF-1alpha with the Lipofectamine 2000 system. The positive clone cells were selected by G418 and confirmed by Western blotting and immunofluorescence (LNCaP/HIF-1alpha cells). The expressions of VEGF, iNOS and Ang-2 were detected by Western blotting.
RESULTSThe expression of HIF-1alpha in the LNCaP/HIF-1alpha cells was distinctly higher than that in the LN-CaP cells. Compared with the LNCaP cells, the expressions of VEGF and iNOS were up-regulated, whereas Ang-2 remained unchanged in the LNCaP/HIF-1alpha cells.
CONCLUSIONThe over-expression of HIF-1alpha can induce an increase in angiogenesis proteins and improve the angiogenesis potency of prostate cancer.
