Two-dimensional gel electrophoresis profiles of human normal prostate cells successfully established by 2-DE coupled with laser capture microdissection.
- Author:
Qun LI
1
;
Yi-Lei LI
;
Xin-Yu YANG
;
Hai-Ying ZHANG
;
Hui-Min LI
;
Yu-Lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cells, Cultured; Electrophoresis, Gel, Two-Dimensional; methods; Humans; Lasers; Male; Microdissection; methods; Middle Aged; Prostate; cytology; metabolism; Proteome; analysis; Proteomics; methods
- From: National Journal of Andrology 2007;13(12):1064-1067
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a technological system of proteomics research for displaying the full-scale protein expression spectrum of the prostate cells.
METHODSWe obtained the epithelial cells from normal prostate tissues by laser capture microdissection (LCM) technology, extracted the proteins from them and established two-dimensional gel electrophoresis (2-DE) profiles of the proteins by immobilized pH gradient (IPG) two-dimensional electrophoresis. Then protein spot detection and matching were performed with the scanner and the ImageMaster 2D Elite analysis software. With the help of the Ettanspotter and digester, protein spots that matched well across the gels were extracted, digested and further identified by assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
RESULTSThe 2-DE profiles of prostate cells were established, and one protein spot from normal prostate cells identified successfully.
CONCLUSIONThe 2-DE profiles of normal prostate cells have good reproducibility. We have tentatively established the proteomics research technology of normal prostate cells and demonstrated the feasibility of proteomics research with the model of human normal prostate cells.
