Epidermal growth factor up-regulates the mRNA expression of endothelin-1 and its receptors in prostate cancer PC-3 cell lines.
- Author:
Rui-Peng JIA
1
;
Yan-Fei JIANG
;
Lu-Wei XU
;
Shu-Kui WANG
;
Zi-Zheng WANG
;
Wen-Cheng LI
;
Bang-Shun HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Endothelin-1; genetics; Epidermal Growth Factor; pharmacology; Gene Expression Regulation, Neoplastic; drug effects; Humans; Male; Prostatic Neoplasms; genetics; pathology; RNA, Messenger; genetics; metabolism; Receptor, Endothelin A; genetics; Receptor, Endothelin B; genetics; Receptors, Endothelin; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: National Journal of Andrology 2008;14(1):15-19
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of the epidermal growth factor on the mRNA expression of endothelin-1 and its receptors (ET(A)R, ET(B)R) in hormone refractory prostate cancer (HRPC) PC-3 cell lines.
METHODSPC-3 cells were cultured in vitro. After the treatment with EGF, the mRNA expressions of endothelin-1, ET(A)R and ET(B)R were detected by RT-PCR in PC-3 cell lines. The levels of the mRNA expression of endothelin-1 and its receptors were examined at different time points by RT-PCR.
RESULTSThe expressions of endothelin-1 and ET(A)R mRNA but not the mRNA expression of ET(B)R was observed in PC-3 cell lines. After 24 hours of treatment with EGF, the expressions of endothelin-1 and ET(A)R in PC-3 cell lines were both up-regulated and there was significant difference (P < 0.05) between the experimental and control groups. Different expression levels of endothelin-1 and ET(A)R mRNA were noted at different time points of EGF intervention, up-regulated with the increase of treatment time, and with significant difference (P < 0.05).
CONCLUSIONEGF can up-regulate the mRNA expressions of endothelin-1 and ET(A)R in PC-3 cell lines and play a great role in prostate cancer progression, which may offer a substructure of molecular biology for the treatment of HRPC.
