Effects of two different cryoprotectants on the motility of post-thaw human sperm.
- Author:
Jing-Mei HU
1
;
Cheng-Yan XU
;
Yuan LI
;
Shao-Ming LU
;
Li WANG
;
Zi-Jiang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cryopreservation; methods; Cryoprotective Agents; pharmacology; Glucose; pharmacology; Humans; Male; Semen Preservation; methods; Sperm Motility; drug effects; Spermatozoa; drug effects; physiology; Sucrose; pharmacology
- From: National Journal of Andrology 2008;14(1):23-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm.
METHODSThe cryoprotectant containing glucose and that containing sucrose were applied to 50 semen samples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups.
RESULTSThe forward motility and total motility of the sperm were (58.4 +/- 5.7)% and (63.4 +/- 6.1)% before cryopreservation, (43.8 +/- 7.6)% and (48.4 +/- 7.6)% after thawing with the cryoprotectant containing glucose, and(42.6 +/- 8.9)% and (48.0 +/- 8.5)% after thawing with the cryoprotectant containing sucrose. Decreased sperm motility was observed after cryopreservation, with statistic significance (P < 0.01). There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryoprotectants.
CONCLUSIONCryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.
