Enhanced action of a BTB/POZ domain protein on the expression of hsp90alpha gene in heat shock.
- Author:
Yong-jun CHEN
1
;
Zhi-cheng MO
;
Jing ZHANG
;
Ye ZHANG
;
Ning-hua WU
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Motifs; Animals; Checkpoint Kinase 1; Cloning, Molecular; DNA; metabolism; DNA, Complementary; metabolism; DNA-Binding Proteins; metabolism; Drosophila; genetics; Drosophila Proteins; genetics; Gene Expression Regulation; HSP90 Heat-Shock Proteins; biosynthesis; genetics; Heat-Shock Response; genetics; Homeodomain Proteins; chemistry; genetics; metabolism; Humans; Repressor Proteins; genetics; Transcription Factors; genetics; Two-Hybrid System Techniques
- From: Acta Academiae Medicinae Sinicae 2004;26(4):392-395
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of a BTB/POZ domain protein in the expression of hsp90alpha gene.
METHODSThe eukaryotic expression plasmids of sense- and antisense-GAGA related protein (GRP) or empty vector were transfected into Jurkat cells with pREP4 episomal vector plasmids carrying the hsp90alpha promoter sequence from -1756 to +37 and control plasmids pMCAT. Total RNA was extracted. The relative promoter activity of hsp90alpha-CAT reporter gene was determined by competitive RT-PCR assay.
RESULTSGRP markly increased the relative promoter activity of hsp90alpha-CAT reporter gene during heat shock.
CONCLUSIONGRP may promote the expression of hsp90alpha gene by participating in chromatin remolding.
