Effects of 1,25-dihydroxyvitamin D3 on the expressions of osteoprotegerin and receptor activator of NF-kappaB ligand in mouse osteoblasts.
- Author:
Qing-xian TIAN
1
;
Gong-yi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Calcitriol; pharmacology; Carrier Proteins; biosynthesis; genetics; Glycoproteins; biosynthesis; genetics; physiology; Ligands; Membrane Glycoproteins; biosynthesis; genetics; Mice; NF-kappa B; biosynthesis; genetics; Osteoclasts; metabolism; physiology; Osteoprotegerin; RANK Ligand; RNA, Messenger; biosynthesis; genetics; Receptor Activator of Nuclear Factor-kappa B; Receptors, Cytoplasmic and Nuclear; analysis; biosynthesis; genetics; physiology; Receptors, Tumor Necrosis Factor; biosynthesis; genetics; Tumor Necrosis Factor-alpha; biosynthesis; genetics
- From: Acta Academiae Medicinae Sinicae 2004;26(4):418-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on the expression of osteoprotegerin (OPG) and receptor activator of NF-kappaB ligand (RANKL) mRNA in mouse osteoblasts.
METHODSCalvariae derived from CD-1 neonatal mouse (after born 24 h). Bone samples were processed by the collagenase/trypsin digestion method. Mouse osteoblasts were cultured in vitro. After 48 hours of addition of 1,25(OH)2D3 (0, 10(-8), 10(-9), 10(-11) mol/L) to the culture medium of mouse osteoblasts, the content of the OPG protein in culture medium was estimated with enzyme linked immunosorbent assay. Total RNA was prepared from mouse osteoblasts. mRNA expression of OPG and RANKL were detected by reverse transcription-polymerase chain reaction.
RESULTSThe mRNA expression of OPG in osteoblasts added with 1,25(OH)2D3 significantly decreased compared with the controls, which was markedly dose-dependent. OPG protein production in the medium decreased after treatment with 1,25(OH)2D3. In contrast, RANKL mRNA expression levels in osteoblasts significantly increased after 48 h of culture with 1,25(OH)2D3.
CONCLUSION1,25 (OH)2D3 can stimulate RANKL mRNA expression, but decrease OPG mRNA levels in vitro in mouse osteoblasts.
