Role of PD153035 in the induction of apoptosis of XG-1 myeloma cell line.
- Author:
Xiao-yan HE
1
;
Jian-an HUANG
;
Wei XIE
;
Ling-yu JIANG
;
Yue-dan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Cell Division; Cell Line, Tumor; DNA-Binding Proteins; metabolism; Humans; Multiple Myeloma; pathology; Quinazolines; pharmacology; Receptor, Epidermal Growth Factor; antagonists & inhibitors; STAT3 Transcription Factor; Trans-Activators; metabolism
- From: Acta Academiae Medicinae Sinicae 2004;26(5):492-495
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of epidermal growth factor receptor (EGFR) in the proliferation and survival of human myeloma cells.
METHODSThe inhibitor of EGFR, PD153035, was used to block the signal transduction of EGFR. The proliferation and apoptosis of myeloma cell line, XG-1, were detected by 3H-TCR incorporation assay and Annexin V staining analysis, respectively. The phosphatation of STAT3, the key activate signal to the myeloma cell proliferation, was detected with Western blot.
RESULTSPD153035 decreased the proliferation of XG-1 and induced an obvious apoptosis in XG-1. The phosphatation of STAT3 induced by HB-EGF but not by IL-6 was blocked by PD153035.
CONCLUSIONThe proliferation and survival of myeloma cells may be suppressed by PD153035 due to the blockage of phosphatation of STAT3 induced by the activation of EGFR.
