Genetic association between interleukins gene polymorphisms with primary biliary cirrhosis in Chinese population.
- Author:
Lie-ying FAN
1
;
Ye ZHU
;
Ren-qian ZHONG
;
Xiao-qing TU
;
Wei-min YE
;
Qu-bo CHEN
;
Wan-jie ZENG
;
Xian-tao KONG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Humans; Interleukin-1; genetics; Interleukin-10; genetics; Interleukin-6; genetics; Liver Cirrhosis, Biliary; genetics; Male; Middle Aged; Polymerase Chain Reaction; methods; Polymorphism, Restriction Fragment Length
- From: Acta Academiae Medicinae Sinicae 2004;26(5):505-509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the relationship between polymorphisms in the genes encoding IL-1, IL-6, and IL-10 with primary biliary cirrhosis (PBC) in Chinese population.
METHODSWhole-blood samples were taken from 77 patients with PBC and 160 healthy controls. DNA was extracted and the polymorphisms at positions IL-1 +3953, IL-1RN intron 2, IL-6 -174, and IL-10 -1082, -819, and -592 were determined by using sequence-specific polymerase chain reaction (SSP) or polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
RESULTSThe frequency of IL-1RN1,1 allele in PBC group was significantly higher than in control group (90.9% vs 79.4%, P=0.026), and the frequency of IL-1RN1,2 in PBC group was significantly lower than in control group (6.5% vs 18.8%, P=0.013). There was no significant difference in the frequence of IL-1RN*2 allele between PBC group and control group (P=0.06). Of the 77 patients with PBC, 4 patients were IL-6 -174GC, 73 were IL-6 174GG. All the 160 health controls are IL-6 -174GG (P=0.0036). The frequence of IL-6 -174C allele in PBC group was significantly higher than that in control group (P=0.0038). No significant differences of polymorphisms for IL-1 +3953 and IL-10 (-1082, -819 and -592) were found between PBC group and control group.
CONCLUSIONThe polymorphisms of IL-1RN and IL-6 -174G/C appear to be associated with PBC, and the polymorphisms of IL-1 +3953 and IL-10 promoter gene are not associated with PBC in a Chinese population.