Regulation of Fc receptor expression by immune complexes on neutrophils and U937 cells.
- Author:
Li-hua GUO
1
;
Wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Anti-Idiotypic; immunology; Antibodies, Monoclonal; pharmacology; Antigen-Antibody Complex; immunology; metabolism; Antigens, CD; immunology; Humans; Immunoglobulin A; classification; immunology; Immunoglobulin G; chemistry; classification; immunology; metabolism; Neutrophils; metabolism; Receptors, Fc; biosynthesis; genetics; Receptors, IgG; immunology; U937 Cells; immunology
- From: Acta Academiae Medicinae Sinicae 2004;26(5):510-514
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the regulation of Fc receptor expression by immune complexes (ICs) on neutrophils and U937 cells.
METHODSIgA ICs, IgG1 ICs, IgG2 ICs, IgG3 ICs, IgG4 ICs, and IgM ICs were incubated with neutrophils or U937 cells for 1 h. Then their surface Fc receptors were stained by anti-Fc gammaR I, anti-Fc gammaR II , anti-Fc gammaR III, and anti-Fc alphaR I monoclonal antibodies and analyzed by fluorescent activated cell sorting (FACS).
RESULTSIgG1 ICs and IgG3 ICs up-regulated Fc gammaR II and Fc gammaR III on U937 cells, Fc gammaR I and Fc alphaR I on neutrophils. Almost all ICs down-regulated Fc gammaR II on neutrophils.
CONCLUSIONSICs can regulate Fc receptor expression on neutrophils and U937 cells, among which IgG1 ICs and IgG3 ICs are most effective.
