Cloning and expression of a homologue of human macrophage migration inhibitory factor from P. falciparum 3D7.
- Author:
Zhi-fu HAN
1
;
Ding-ding SHAO
;
Heng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Escherichia coli; genetics; Humans; Macrophage Migration-Inhibitory Factors; biosynthesis; genetics; isolation & purification; Molecular Sequence Data; Plasmodium falciparum; genetics; metabolism; Protozoan Proteins; biosynthesis; genetics; isolation & purification; Recombinant Proteins; biosynthesis; genetics; Sequence Homology, Amino Acid
- From: Acta Academiae Medicinae Sinicae 2004;26(5):515-518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone and express a homologue of human macrophage migration inhibitory factor (MIF) from P. falciparum 3D7--PfMIF.
METHODSThe nucleotide sequence of PfMIF was found through blast P. falciparum genomic sequence databases with the amino acid sequence of human MIF (HuMIF). RT-PCR, DNA sequencing, and bioinformatics analysis were used for the cloning of Pfmif gene. The recombinant protein was expressed in E. coli and purified through the affinity column.
RESULTSThe full length of Pfmif gene was cloned and sequenced. It was composed of 351 nucleotides and encoded 116 amino acids with the typical characteristic of MIF family. The recombinant protein was successfully expressed and purified.
CONCLUSIONSThe Pfmif gene and recombinant protein were successfully isolated and PfMIF was preliminarily identified as a novel member of MIF family.
