Establishment of immortalized cell line BLTR-4 and primary identification of its biological character.
- Author:
Shan ZHENG
1
;
Su-ping GUO
;
Zu-gen HE
;
Shu-jun CHENG
;
Yan-ning GAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Transformed; Humans; Oncogene Proteins, Viral; genetics; Papillomaviridae; genetics; Papillomavirus E7 Proteins; Papillomavirus Infections; virology; Plasmids; genetics; Repressor Proteins; genetics; Transcription, Genetic; Transfection; Tumor Virus Infections; virology; Urinary Bladder; cytology; Urinary Bladder Neoplasms; virology
- From: Acta Academiae Medicinae Sinicae 2004;26(5):543-548
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish immortalized cell line from the urothelium of the urinary bladder and identify the characteristics of the cell line.
METHODSHuman papillomavirus 16 (HPV-16) plasmid was used to transfect urothelium of infant urinary bladder in vitro with the help of Fugene-6, and this plasmid contained E6 and E7 genes of HPV-16. We also identified the existence of HPV-16 E6 and E7 genes and the biological characteristics of the cell line by PCR, immunohistochemistry, and the biology identification.
RESULTSBLTR-4 cell line, produced from the transfection of HPV-16K plasmid, was a cell line from urothelium with the expression of HPV-16 E6 and E7 genes. It had been cultured more than 70 passages, and the characteristics of growth was similar to the immortalized cell line as reported.
CONCLUSIONSBLTR-4 cell line is an immortalized cell line from urothelium of the urinary bladder, which contains HPV-16 E6 and E7 genes. BLTR-4 cell line is a good experimental model to investigate the relationship of the infection of high risk HPV and transitional cell carcinoma (TCC) in vitro.
