Application of quantitative evaluation on multicomponent drug interaction in anti-HIV research.
- Author:
Pei-Zhi DONG
1
;
Yong-Xiang GAO
1
;
Xian-Bo WU
1
;
Guo-Dong CEN
1
Author Information
- Publication Type:Journal Article
- MeSH: Antiviral Agents; administration & dosage; pharmacology; Cell Line; Dose-Response Relationship, Drug; Drug Antagonism; Drug Synergism; HIV-1; drug effects; Humans
- From: Chinese Journal of Integrated Traditional and Western Medicine 2013;33(12):1665-1667
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo quantitatively evaluate mutual relations of 4 component drugs in anti-HIV action.
METHODSThe effect of TCM four components on cell growth was detected using MTT assay. The antiviral effects of 4 components were observed at the maximal nonvenomous dose. The combination index (CI) value of combined two or four components were calculated using median-effect principle. The mutual relations of two or four components for antiviral actions were assessed using CI.
RESULTSSynergism was dominant in combination of A and B, and the effect was dose-dependent. Antagonism was dominant in combination of C and D, and the effect was dose-dependent. But the combination of A, B, C, and D was synergistic when the inhibition rate was over 10%.
CONCLUSIONMedian-effect principle can be used to quantitatively assess the anti-HIV effect of four components.
