Effect of Qingyi Decoction on gene expression profiles of severe acute pancreatitis rats by gene chip technique.

Feng-Shang ZHU; Guo-Ying ZHU; Dong-Ping HUANG; Xiao-Ying SHEN; Chang-Qing YANG; Heng-Jun GAO

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Effect of Qingyi Decoction on gene expression profiles of severe acute pancreatitis rats by gene chip technique.

Author: Feng-Shang ZHU ¹ ; Guo-Ying ZHU ² ; Dong-Ping HUANG ² ; Xiao-Ying SHEN ³ ; Chang-Qing YANG ¹ ; Heng-Jun GAO ¹
Author Information

1. Department of Gastroenterology, Tongji Hospital of Tongji University, Shanghai 200065, China.
2. Office of Hospital, Shanghai Putuo District People's Hospital, Shanghai 200060, China.
3. Shanghai National Engineering Center for BioChip, Shanghai 201203, China.
Publication Type:Journal Article
MeSH: Animals; Drugs, Chinese Herbal; therapeutic use; Female; Gene Expression Profiling; Male; Oligonucleotide Array Sequence Analysis; Pancreatitis; drug therapy; genetics; Phytotherapy; Rats; Rats, Sprague-Dawley; Transcriptome
From: Chinese Journal of Integrated Traditional and Western Medicine 2014;34(1):51-55
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effect of Qingyi Decoction (QYD) on pancreatic gene expression profiles in rats with severe acute pancreatitis (SAP).
METHODSTotally 60 Sprague-Dawley (SD) rats were randomly divided into the sham-operation group (SO group), the SAP group, and the QYD group, 20 in each group. SAP model was replicated by the pancreatic duct retrograde injection with 4% sodium taurocholate. Rats in the QYD group was intragastrically intervened by QYD (0.75 mL/100 g) for 3 times. Pancreatic RNA expression was analyzed using Illuminate whole genome expression profiles. Changes of mRNA and protein in specific genes [heat shock proteins a8 (Hspa8) and heat shock proteins b1 (Hspb1)] were verified by real-time quantitative PCR and Western blot analysis.
RESULTSCompared with the SAP group, 575 differential genes were screened in the QYD group, including 92 up-regulated genes and 483 down-regulated genes. Gene Ontology (GO) categories indicated the genes are associated with negative regulation of transcription regulator activity, oxidoreductase activity and enzyme inhibitor activity. Effects of QYD on the SAP rats were major related to mitogen-activated protein kinase (MAPK), NOD like receptors (NLR) receptor-like signaling pathway, cell cycle, metabolic pathways, oxidoreductase activity. Protein and mRNA changes of Hspa8 and Hspb1 in microarray were verified [relative mRNA expression for Hspa8 and Hspb1 was increased by (13.24 +/- 1.22) times and (7.55 +/- 1.09) times respectively, P < 0.01].
CONCLUSIONQYD was effective in treating experimental SAP involved the MAPK and NLR signaling pathways, cell cycle, metabolic pathways, and oxide reductase activities.