The inhibitory effect of matrine on the growth of human colorectal cancer HT29 cells: an experimental observation.
- Author:
Cheng CHANG
1
;
De-Li RAO
1
;
Xiao-Ming QIU
1
;
Hong WANG
1
;
Li XIONG
2
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Apoptosis; drug effects; Cell Proliferation; drug effects; HT29 Cells; Humans; Quinolizines; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2014;34(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory and inductive effect of matrine (MA) on human colorectal cancer HT29 cells.
METHODSMTT assay was used to determine the cell growth inhibitory rate in vitro . Changes of cell cycle and the apoptosis of HT29 cells before and after MA treatment were observed using flow cytometry and electron microscope.
RESULTSMTT showed that 2-32 mg/mL MA inhibited the proliferation of HT29 cells (P < 0.05) in a dose- and time-dependent manner. Cells treated by 4, 8, and 16 mg/mL MA at G0/G1 phase were obviously higher than those in the negative control group (P < 0.05), indicating that the cell cycle was arrested at G0/G1 phase. Morphological apoptosis of HT29 cells could be seen under transmission electron microscope.
CONCLUSIONMA inhibited the proliferation of HT29 cells, and its mechanism might be associated with stagnation at G0/G1 phase and inducing apoptosis of HT29 cells.