Effect of bone morphogenetic protein-7 on aristolochic acid induced renal tubular epithelial cells transdifferentiation.
- Author:
Yong-zhi XU
1
;
Hao HUANG
;
Hua-feng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Aristolochic Acids; adverse effects; Bone Morphogenetic Protein 7; pharmacology; Cell Line; Cell Transdifferentiation; drug effects; Epithelial Cells; cytology; Humans; Kidney Tubules, Proximal; cytology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(2):157-160
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of bone morphogenetic protein-7 (BMP-7) on aristolchic acid induced renal tubular epithelial cell trans-differentiation to look for new therapeutic approach for aristolchic acid nephropathy (AAN).
METHODSIn vitro cultured human proximal renal tubular epithelial cell line HK-2 cells were treated with different concentrations of BMP-7 (75 ng/mL, 150 ng/mL and 300 ng/mL) after trans-differentiation of the cells was induced by AA (10 microg/mL). Levels of alpha-SMA mRNA and protein expressions were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively.
RESULTSBMP-7 reversed the AA inducing alpha-SMA expressions in HK-2 cells in a dose-dependent manner.
CONCLUSIONBMP-7 can inhibit the trans-differentiation of human renal tubular epithelial cell induced by AA, thereby might be a new potential drug for AAN prevention and treatment.
