Screening, identification and analysis of platelet differential functional proteins in patients with coronary heart disease of blood-stasis pattern.
- Author:
Xue-Feng LI
1
;
Yue-Rong JIANG
;
Zhu-Ye GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Blood Platelets; Case-Control Studies; Coronary Disease; blood; diagnosis; physiopathology; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Male; Medicine, Chinese Traditional; Middle Aged; Platelet Membrane Glycoproteins; isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(5):467-473
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo seek the key platelet functional proteins (PFPs) for the occurrence of blood-stasis pattern (BP) in patients with coronary heart disease (CHD).
METHODSPeripheral blood platelet protein of 22 patients and 24 healthy person (for control) were extracted respectively in 4 batches for carrying 4 times of the test out. Differential PFPs in samples were screened out by two-dimensional fluorescence difference gel electrophoresis, and identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry; then the identified proteins were further authenticated by Western-blotting.
RESULTSThirteen differential PFPs were screened out, and among them the 7 identified by spectrometry were: isoform 1 of integrin alpha- II b, isoform 2 of integrin alpha- II b, actin-cytoplasmic 1, actin-cytoplasmic 2, cDNA FLJ52842, cDNA FLJ55253, and cDNA FLJ43573 fis. Among them isoform 2 of integrin alpha- II b (CD41) and actin-cytoplasmic 2 (Acting) were authenticated successfully.
CONCLUSIONCD41 and acting are the possible marker proteins, and the other PFPs might play crucial roles in the occurrence and development of BSS in CHD.
