Gel electrophoresis analysis on plasma differential protein in patients with unstable angina of blood-stasis pattern.
- Author:
Hui-Hui ZHAO
1
;
Jian-Xin CHEN
;
Qi SHI
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Angina, Unstable; blood; diagnosis; Apolipoprotein A-II; blood; Apolipoprotein C-III; blood; Blood Proteins; metabolism; Case-Control Studies; Female; Fibrinogen; Humans; Male; Medicine, Chinese Traditional; Middle Aged; Proteome; analysis; Proteomics; Two-Dimensional Difference Gel Electrophoresis
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(5):488-492
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo seek the plasma differential proteins in patients with unstable angina of blood-stasis pattern (UA-BSS) for exploring the proteomic specialty in them by way of two-dimensional difference gel electrophoresis (DIGE) detection on plasma of patients and healthy persons.
METHODSUsing DIGE and tandem mass spectrometry, comparative proteomic study was conducted on the plasma of 12 UA patients of qi-deficiency and blood-stasis pattern (UA-QBS), 12 UA patients of phlegm-stasis cross-blocking pattern (UA-PSS) and 12 healthy volunteers.
RESULTSPreliminary results showed that Haptoglobin beta chain, DBP, HBB, HBA, Transthyretin, ApoA- I, ApoA-IV were significantly differentially expressed in both patterns, while Haptoglobin alpha1 chain, alpha-1-acid glycoprotein, ApoC-III, ApoA-II, ApoC-II, ApoJ, and Haptoglobin alpha 2 chain were only seen differentially expressed in the UA-PSS patients, alpha1-antitrypsin, Fibrinogen gamma chain, and Fibrin beta were only seen differentially expressed in UA-QBS patients.
CONCLUSIONThe common proteomics characteristics of patients of QBS and PSS patterns may be correlated with inflammatory reaction and metabolic disturbance (including blood lipid and blood oxygen).
