Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli.
- Author:
Ji-Bin YU
1
;
Ping JI
;
Xin-Min ZHA
;
Wei-De SHEN
;
Xiang-Fu WU
Author Information
1. College of Life Sciences, Suzhou University, Suzhou 210006, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cloning, Molecular;
Escherichia coli;
genetics;
Gene Expression;
drug effects;
Isopropyl Thiogalactoside;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
Scorpion Venoms;
biosynthesis;
chemistry;
genetics;
Scorpions;
chemistry;
genetics
- From:
Chinese Journal of Biotechnology
2002;18(1):106-108
- CountryChina
- Language:Chinese
-
Abstract:
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
