Extraction of the salt-tolerant cDNA in mangrove Avicennia marina by mRNA differential display.
- Author:
Han-Tao ZHOU
1
;
Peng LIN
Author Information
1. School of Life Sciences, Xiamen University, Xiamen 361005, China. htzhou@public.xm.fj.cn
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cloning, Molecular;
DNA, Complementary;
isolation & purification;
DNA, Plant;
Gene Expression Profiling;
methods;
Gene Expression Regulation, Plant;
drug effects;
Magnoliopsida;
Maximum Tolerated Dose;
Plant Leaves;
genetics;
physiology;
Salts;
pharmacology;
Sequence Analysis, DNA;
methods
- From:
Chinese Journal of Biotechnology
2002;18(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
RNAs of the leaves in Avicennia marina, which cultured in 50@1000 and 0@1000 salinity condition respectively, were isolated for mRNA differential display analysis. Screened by OligodT12 GC and eight 10-oligonucleotide arbitrary primers, differential cDNA fragments-csrg1(600 bp), csrg2(550 bp), csrg3(480 bp), only appeared in the leaves of Avicennia marina cultured in 50@1000 salinity condition. After detected by RNA dot hybridization, only csrg1 appeared the difference between the RNAs of the leaves in Avicennia marina cultured in 50@1000 and 0@1000 salinity condition respectively, and csrg1 was confirmed as the salt-tolerant cDNA. csrg1 was cloned and sequenced. After searching in Genbank, there were no similar sequences reported.
