Visualization of the interaction between IL-6 and IL-6R by imaging ellipsometry.
- Author:
Zhan-Hui WANG
1
;
Gang JIN
Author Information
1. Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Diagnostic Imaging;
instrumentation;
methods;
Humans;
Interleukin-6;
metabolism;
Microscopy, Polarization;
methods;
Receptors, Interleukin-6;
metabolism;
Surface Properties
- From:
Chinese Journal of Biotechnology
2002;18(1):99-101
- CountryChina
- Language:Chinese
-
Abstract:
Imaging Ellipsometry is one of recently developed optical surface-sensitive methods for the investigation of various aspects of biomolecules adsorption on solid surfaces and biomolecules interactions. It has advantages of high sensitivity to layer-thickness, big area of view, high sampling speed, and high lateral resolution. Compared with other solid phase methods such as enzyme linked immunosorbent assay, immunofluorescence and radioimmunoassay, imaging ellipsometry has the advantage of not involving any labelling of reactants and it is a relatively inexpensive method and easy to handle. In this report, the mono-layers of IL-6 and IL-6 receptor were visualized as well as their interaction.
