Study on the expression of E2 gene of classical swine fever virus in Pichia pastoris and the immunological activity of its expression product.
- Author:
Xue-Qing HAN
1
;
Xiang-Tao LIU
;
Yong ZHANG
;
Qing-Ge XIE
;
Bo TIAN
Author Information
1. Lanzhou Veterinary Research Institute, CAS, Lanzhou 730046, China. hanxq8@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
immunology;
Antigens, Viral;
genetics;
immunology;
Classical swine fever virus;
genetics;
immunology;
Cloning, Molecular;
Gene Expression;
Pichia;
Rabbits;
Swine;
Viral Envelope Proteins;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2002;18(2):208-211
- CountryChina
- Language:Chinese
-
Abstract:
E2 gene of classical swine fever virus (CSFV) was cloned into secretory pPIC9K Pichia pastoris expression vector. After being linearized by digestion, the vector was transformed into Pichia pastoris by electroporation to integrate with the genome, the transformants with high copies were screened by G418 and were induced to express with methonal. The results of SDS-PAGE and Western blot demonstrated that the supernatant of the induced P. pastoris culture contained protein E2. The results of the study on the immunological activity indicated that the protein E2 expressed in P. pastoris can elicit animal bodies to produce antibodies against protein E2.
