Lipase-catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester in organic solvent.
- Author:
Wei DU
1
;
Min-Hua ZONG
;
Yong GUO
;
Jun HE
;
Yuan-Yuan ZHANG
;
Zhao-Lin XIE
;
Wen-Yong LOU
Author Information
1. Biotechnology Department, South China University of Technology, Guangzhou 510640, China. crystal-du@263.net
- Publication Type:Journal Article
- MeSH:
Alcohols;
Ammonia;
Catalysis;
Dimethylformamide;
pharmacology;
Esters;
Glycine;
analogs & derivatives;
metabolism;
Hexoses;
pharmacology;
Hydrolysis;
Lipase;
drug effects;
metabolism;
Organic Chemicals;
Solvents;
Surface-Active Agents;
pharmacology;
Temperature;
Water
- From:
Chinese Journal of Biotechnology
2002;18(2):242-245
- CountryChina
- Language:Chinese
-
Abstract:
A novel reaction-enzymatic ammonolysis discovered in the mid of 1990s has been demonstrated to be a very promising alternative in the preparation of optically pure compounds. The effects of organic solvent, initial water activity, temperature and additives on lipase Novozym435-catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester were investigated systematically in this paper. Enzymatic reaction of ammonolysis showed higher activity and enantioselectivity than the corresponding reaction of hydrolysis and alcoholysis.
