RT-PCR identification and genetic characterizations of VP1 region of human enterovirus 71 isolated in Beijing in 2008.
- Author:
Dian HE
1
;
Rui-Ting BAI
;
Yong ZHANG
;
Zhi-Lan SHAN
;
Li LI
;
Jie LIU
;
Jian-Xin MA
;
Xiao-Xi ZHANG
;
Dong-Mei YAN
;
Wen-Bo XU
Author Information
- Publication Type:Journal Article
- MeSH: Capsid Proteins; genetics; China; epidemiology; Disease Outbreaks; Enterovirus A, Human; genetics; isolation & purification; Enterovirus Infections; epidemiology; genetics; Hand, Foot and Mouth Disease; epidemiology; virology; Humans; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; methods
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(6):421-423
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic characterizations of VP1 region of Human enterovirus 71 (HEV71) isolated from clinical specimens of hand, foot and mouth disease (HFMD) patients in Beijing in 2008.
METHODS285 clinical samples were collected from HFMD patients in hospitals and day-care centers in Chaoyang district. They were performed by reverse transcription-polymerase chain reaction (RT-PCR) specific for HEV71. 10 HEV71 isolates were selected for entire VP1 coding gene amplification and sequencing.
RESULTS129 samples were RT-PCR positive, the positive rate is 45.26%. The homology of the nucleotide and the amino acid of the 10 strains were 94.6%-99.6% and 95.9%-100%. The phylogenetic tree revealed that 10 Beijing strains clustered within the C4a evolution branch of C4 subgenotype.
CONCLUSIONSRT-PCR played an important role in identifying HFMD outbreak in Beijing in 2008. The HEV71 strains were all belong to C4a evolution branch of C4 subgenotype with several transmission chains, and it showed that C4 subgenotype HEV71 spread in mainland China widely after 1998. The molecular epidemiology surveillance and the research of genetic characterizations of HEV71 should be strengthened in mainland China.
