Incidence and relationship of human cytomegalovirus infection and human herpesvirus 6 infection in pediatric patients after hemopoietic stem cell transplantation
10.3760/cma.j.issn.1003-9279.2010.06.020
- VernacularTitle:儿童造血干细胞移植患者人巨细胞病毒与疱疹病毒6型感染及相互关系
- Author:
Dian-Yi LV
1
;
Mao-Quan QIN
;
Yan WANG
;
Li-Wei GAO
;
Zheng-De XIE
Author Information
1. 首都医科大学附属北京儿童医院
- Keywords:
Hematopoietic stem cell transplantation;
Cytomegalovirus;
Herpesvirus 6,human
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(6):455-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the incidence of human cytomegalovirus (CMV) and human herpesvirus 6(HHV-6) infection in pediatric patients with hemopoietic stem cell transplantation (HSCT),and to explore the relationship between CMV and HHV-6 infection in pediatric patients with HSCT.Methods Pediatric patients with HSCT in hemotology center of Beijing Children's Hospital were enrolled into this study from June 2007 to October 2009. Peripheral blood were collected every week after HSCT, and Fluorescent quantitation PCR and conventional PCR were used to detect CMV DNA load in serum and HHV-6 DNA in peripheral blood respectively. Genetic typing was conducted on HHV-6. Results Fifty two pediatric patients with HSCT were enrolled into this study, and six hundreds and thirty six specimens were collected totally. CMV DNA was detected in fifty two specimens from twenty cases. The median time was 56days after HSCT. The incidence of CMV infection was 38.5% (20/52)in all HSCT patients and 47. 6% (20/42) in allogene HSCT patients. The incidence of late CMV infection was 22.2% (6/27)in allogene HSCT.Three patients died of C MV infection,and two died of CMV interstitial pneumonia. HHV-6 DNA was detected in thirty three specimens from fourteen cases. The median time was 23 days after HSCT. The incidence of HHV-6 infection was 26.9% ( 14/52 ) in all HSCT patients and 31% ( 13/42 ) in allogene HSCT patients. The genotype of HHV6 was all type B. HHV-6 DNA was positive in six of twenty cases with CMV infection. The
