Clinical application Studies on AFP-L3 detected by micro-spin column method
10.3760/cma.j.issn.1003-9279.2010.06.022
- VernacularTitle:微量离心柱法检测AFP-L3的临床应用研究
- Author:
Jin GAO
1
;
Ai-Fang XU
;
Hong-Ying ZHENG
;
Gang CHEN
;
Miao-Chan WANG
;
Guo-Qiang LOU
Author Information
1. 杭州市第六人民医院
- Keywords:
Alpha-fetoproteins;
Carcinoma,hepatocellular;
Forecasting;
Diagnosis,differential
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(6):461-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical value of Lens culinaris agglutinin-reactive alphafetoprotein in the differentiation diagnosis between benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma. Methods Alpha-fetoprotein variants from 300 patients with liver diseases were isolated with micro-spin column equipped lens culinaris agglutinin (LCA). The AFP and AFP-L3 were detected by the electrochemical luminescence (ECL) method, and the proportions of AFP-L3 were calculated. Results The positive rates of AFP-L3 of HCC patients and chronic liver disease patients were 95% and 64% respectively, there were significant difference in two groups (x2 = 134.72, P ＜ 0. 01 ),the HCC incidence rates of AFP-L3 positive and negative chronic liver disease patients showed significant difference(x2 = 80. 158, P ＜ 0.01 ). there were no correlations between the proportion of AFP-L3 and AFP consistency(r = 0.046,P ＞0.05). Conclusions The detection of AFP-L3 by micro-spin column assay show great clinical value in the differentiation diagnosis of benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma.
